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Br J Clin Pharmacol 56:635638

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401 Interestingly, a TRF with equivalent caloric intake from HFD has been shown to have a protective effect on HFD-induced obesity and associated complications by adjusting various signaling pathways and causing rhythmic creatine-mediated thermogenesis, which may further improve nutrient utilization and energy expenditure and reverse excessive daytime sleepiness induced by paraventricular thalamic nucleus (PVT) dysfunction

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Our team generally recommends starting with 2 mL for a 10 mg vial, as it provides a good balance of concentration and measurement simplicity for most applications