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TUNEL assay INS-1 832/3 SNAP/FLAG-hGLP-1R cells seeded onto coverslips were incubated with or without 5 M thapsigargin in vehicle or 100 nM exendin-4-supplemented media for 8 hours before fixation and terminal deoxynucleotidyl transferase dUTP nick-end labelling (TUNEL) assay performed using the DeadEnd TM Fluorometric TUNEL system (Promega) following the manufacturers instructions
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