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Hippocampal Neuron-Enriched Cultures Primary neuronal cultures were established from 18-day-old embryonic rat hippocampi, as previously described ( 2 ) glass coverslips, previously coated with poly(D-lysine) (50 g/ml) and then with DMEM/HAM F12 containing 10% FCS

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Subcutaneous (SQ) injection: A small injection just under the skin may be used in regenerative protocols to enhance deeper repair and collagen remodeling