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G., Bech, P., Dencker, S

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The final 25 L PCR reactions contained 1 L of template, 1X Phusion, High Fidelity Buffer (New England Biolabs), 200 M dNTPs (Promega), 10 nM primers (8 F and 1492 R) and 0.225 units of Phusion DNA Polymerase (New England Biolabs)