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The cells were lysed in lysis buffer [10 mM Tris (pH 7.4), 10 mM KCl, 2 mM MgCl, 1 mM EGTA, 1 mM DTT, 0.05% (v/v) Triton X-100 and 1 protease inhibitor (Pierce)] for 30 min on ice

J Virol 94:e01692-19 Valiya Veettil M, Dutta D, Bottero V, Bandyopadhyay C, Gjyshi O, Sharma-Walia N, Dutta S, Chandran B (2014) Glutamate secretion and metabotropic glutamate receptor 1 expression during Kaposis sarcoma-associated herpesvirus infection promotes cell proliferation