For flow cytometry experiments, cells were prepared as indicated in the flow cytometry section and incubated for 30 min at 37 C with Calcein-AM (10 M) or Rhonox (5 M) before being washed twice with PBS and suspended in PBS containing 0.5% serum prior to being analyzed
References: Chang Y, Huang R, Zhai Y, Huang L, Feng Y, Wang D, Chai R, Zhang W, Hu H
This modification allows SAG to remain intact through the digestive system and be directly absorbed by cells, providing superior bioavailability
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Differential subcellular localization of the RI and RII Na+ channel subtypes in central neurons
Li 2024), pulmonary fibrosis models (Han 2022