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However, molecular biology techniques of targeted mutations using CRISPR/Cas, as well as functional studies of protein production and characterization, with activity assays, in addition to in silico and in vitro studies of protein-ligand or protein-protein interactions, could clarify the specific roles of these motifs (Ltoff et al., 1998

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Mfn1 and Mfn2 regulate the fusion of the outer mitochondrial membrane which occurs earlier, whereas OPA1 is normally associated with the fusion of the inner mitochondrial membrane

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