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The binding reaction was then neutralized with a buffered pH change, and the peptide-HLA complexes were transferred to a 96-well plate coated with a pan-HLA-DR antibody, clone L243 (BioLegend, San Diego, CA, United States), and incubated overnight

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This route fits patients pursuing systemic regenerative protocols, wound healing support, or broader anti-aging programs where systemic distribution is the goal, not routine skin or hair maintenance

The post-needling topical reaches fibroblasts directly through open channels