Briefly, plates were coated overnight with anti-human Fc (Jackson ImmunoResearch Laboratories 109-001-008), blocked for 2 h with PBS 2% BSA at RT, and incubated overnight at 4 C with recombinant Gp1-Fc protein derived from HEK293 cells transfected with LCMV glycoprotein vector
This free radical damage leads to premature skin ageing
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