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In addition, the increased CSFserum leptin ratios in patients with IIH compared with controls suggest that transfer of leptin over the bloodbrain barrier is not impaired in IIH, in contrast to obesity

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Higher-order structure characterisation through techniques such as NMR, circular dichroism (CD), and FTIR Stability studies to assess degradation pathways including oxidation, deamidation, and oligomerisation Importantly, formulation components can interfere with detection (e.g., UV-active excipients), requiring careful method design and sample preparation strategies such as buffer exchange