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Briefly, the complex was assembled in the excess peptides and solubilized in LMNG/CHS and purified using M1 anti-Flag affinity resin and SEC

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For the cysteine reactivity dataset, peptides were searched with a static modification for carboxyamidomethylation (+57.02146 Da) and a differential modification for the IA-DTB probe (+239.1634 Da)

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While FDA-approved GLP-1 injections like Wegovy and Zepbound have demonstrated 15-20% weight loss in clinical trials, GLP-1 patches lack any peer-reviewed evidence showing they deliver therapeutic blood levels or produce meaningful weight loss