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IL-1 ELISA Cortical samples were weighed and homogenized with metal beads in a volume of Cell Lysis Buffer 2 (R&D Systems) according to their weight, using Qiagens TissueLyser II

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10.1016/S0002-9440(10)62288-2

Adverse event (AE) data were collected throughout the study

Users employing this combination typically take melatonin orally 60 to 90 minutes before bed and DSIP 30 to 60 minutes before bed