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All samples were serially diluted in assay buffer in duplicate and incubated with the cells for 30 min at room temperature (RT) before detection with the cAMP dynamic 2 HTRF assay as described previously 72

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Pancrudo J, Shanske S, Coku J, Lu J, Mardach R, Akman O, et al

(2.2) Initiate at 0.6 mg per day for one week

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Furin cleavage exposes the S2 subunit for further processing by the host serine proteases for subsequent viral entry 72,73