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For intracellular cytokine and MMP-9 detection, PC-3, DU-145, LNCaP, BPH, and TNF (10 ng/mL) pre-activated HUVECs (3 10 5 cells/per FACS tube) were fixed and permeabilized, using the CytoFix/Cytoperm kit (Becton Dickinson), and stained with the following PE-conjugated Mabs: anti-human VEGF (Clone #23410, R&D Systems), CXCL12/SDF-1 (Clone #79018, R&D Systems)

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2014;9(8): e104374

Establishment of K7M2-Luc orthotopic osteosarcoma mouse model and therapeutic effect evaluation Tumor model establishment To establish orthotopic model, K7M2 cells (3 10 6 cells/well) were dispersed in PBS buffer and implanted into right tibia of BALB/c mice

A study of 86 patients reported significant improvement in 58.1 percent of cases

Diabetes Care 35 , 25402547 (2012)