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The 6J substrain has a deficiency of the mitochondrial enzyme nicotinamide nucleotide transhydrogenase (NNT), which transfers electron from NADH to NADP + thereby supporting the antioxidant glutathione peroxidase in mitochondria

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GHK-Cu and copper tripeptide-1 refer to the same Gly-His-Lys-Cu(II) molecule

Then, the cells were stained with 4,6-diamidino-2-phenylindole (DAPI) and analysed with BD FACSAria TM III (BD Biosciences)

It acts as a coactivator for a variety of nuclear receptors and transcription factors, participating in the regulation of glucose metabolism, oxidative phosphorylation, overall energy homeostasis, and the genetic program of antioxidant production (Wang et al., 2019b)