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To verify the clinical significance of the hub targets, the expression of the target proteins was verified in the TCGA database, and the affinity between DEHP and 12 key targets (hub targets with p 0.7, active with a probability 0.7, and inactive with a probability 0 were collected for further analysis
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Pull down assay for GPX4 PC12 cells were treated with DAQ probe (DA-yne probe contains FeCl 3 solution) or DMSO for 6 hours, and then were harvested and lysed with RIPA buffer supplemented with protease inhibitor cocktails and phosphatase inhibitor cocktails
