Both media were supplemented with 10% heat-inactivated fetal bovine serum (FBS) and 2 mM glutamine (25030081), all from ThermoFisher Scientific, plus 100 units/mL penicillin (Laboratorios ERN.S.A., Madrid, Spain
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This pathway is responsible of signal transduction across the plasma membrane, via a second messenger, inositol 1,4,5-triphosphate, that modulates intracellular Ca 2+ release as seen in Figure 2