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Gradual Dose Escalation and Monitoring Gradual dose escalation (start low, go slow) allows physiologic adaptation to PT-141 and reduces adverse effects

Evaluation was performed in DiscoverMP (Refeyn Ltd) and molecular masses were determined by matching histogram contrast against a molecular weight standard (Invitrogen/Thermo NativeMark, LC0725)

As a result, cell viability increased from about 40% in the erastin-treated group to nearly 80% at 50 M dopamine, thereby effectively suppressing ferroptosis (129)

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The groups were further divided into four subgroups (n = 5 in each subgroup of the model group and n = 5 in each subgroup of the control group): 1 day, 3 days, 7 days, and 14 days