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Real Time-Polymerase Chain Reaction After drug treatment for 48 h, total RNA of different groups was isolated from middle turn cochlear explants using TRIzol reagent (Life Technologies, 15596026) following manufacturers instructions
Histological examination Histological analysis of the skin was performed by taking 6 mm diameter biopsy punches from areas of interest
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[Google Scholar] Haorah J, Rump TJ, Xiong HG (2013) Reduction of brain mitochondrial betaoxidation impairs complex I and V in chronic alcohol intake: the underlying mechanism for neurodegeneration
Without the ability to store them in tissues, the body can build up enormous IGF reservoirs due to IGFBPs
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