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Briefly, frozen tissue was powdered with a nitrogen-cooled mortar and pestle, and 50-70mg of tissue were homogenized in the absence (for GSH determination) or presence (for GSSG determination) of 1-methyl-2-vinylpyridinium trifluoromethanesulfonate to scavenge reduced thiols

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10.1016/j.pnpbp.2012.09.003 144 StarchenkoG.HrytsykA.RaalA.Koshovyi

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Most protocols suggest: 46 weeks on 13 weeks off before resuming if needed This cycling approach allows healing to continue naturally and may preserve long-term peptide sensitivity