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The muscle data were acquired using a 28 channel knee coil (Quality Electrodynamics [QED], Mayfield Village, USA) or a circular transmit/receive surface coil (10 cm in diameter, Rapid Biomedical, Rimpar, Germany) positioned under the right calf muscle, while the QED coil was unavailable

This analysis included data from four high-quality studies that enrolled 390 participants in total

doi: 10.1038/nm.4000

That is a whopping 14 humans total

Protein extracts (1575 g) were boiled at 95 C for 5 min in loading buffer (100 mM Tris pH 6.8, 10% glycerol, 4% sodium dodecyl sulfate (SDS), 0.2% bromophenol blue and 2 mM -mercaptoethanol) and subjected to 812% SDS polyacrylamide gel electrophoresis (SDS-PAGE) and then transferred to polyvinylidene difluoride (PVDF) membranes