Fascinatingly, this dysbiosis also alters the enzymatic environment of the gut
2.9.3 RT-PCR using SYBR Green Primer testing was conducted using a 25-L reaction mix that contained 12.5 L of the 2x Quanti-Tect PCR SYBR Green Master Mix (Qiagen, Germany), 0.25 L of Revert-Aid Reverse Transcriptase (200 U/L) (Thermo Fisher), 0.5 L of each primer of 20 pmol, 8.25 L of water, and 3 L of RNA template
Wang K, Guo Y, Liu Y, Cui X, Gu X, Li L, et al
Glutathione protects iron homeostasis by controlling the redox state of iron (Fe Fe), supporting ferritin and transferrin function, and preventing iron-driven lipid peroxidation that triggers ferroptosis
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