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doi: 10.1038/icb.2009.88 104 VoglTLeukertNBarczykKStrupatKRothJ

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In brief, HUVECs were lysed in Co-IP buffer containing 20 mM Tris-HCl (pH 7.5), 100 mM NaCl, 0.5 mM EDTA, 0.5% NP-40, 0.5 mM PMSF, and 0.5% protease inhibitor cocktail on ice for 20 min

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Preparation of 20% SBE--CD in Saline (4C, storage for one week): 2 g SBE--CD powder is dissolved in 10 mL Saline, completely dissolve until clear