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You can certainly combine the shots in the same syringe, but I do not recommend it (unless you are an expert), because if you get an air bubble in the syringe, as you extract the liquid from the vial, you will need to slowly return the liquid into the vial and re-extract it again till theres no bubbles

Nature 627, 604611

Make sure there is no air trapped inside and no blood when you pull on the syringe slightly

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Mitochondria also host antioxidant enzymes such as superoxide dismutase (SOD) and catalase as the first line of defense against ROS by converting superoxide to H 2 O 2 and subsequently to water