ZZ: Validation, Writing review & editing
Cells were transiently transfected with 0100 n M siRNA complex using Lipofectamine2000 transfection reagent (Invitrogen, Carlsbad, CA) following the method described earlier (26, 43)
The role of SHBG in the modulation of inflammatory reaction and insulin sensitivity has been finally demonstrated in db/db rodents with overexpressed human SHBG that showed resistance to the development of obesity and hepatosteatosis [32]
Handling should be performed using validated laboratory practices and sterile methodology where appropriate
Cells were pretreated with the indicated inhibitors prior to further treatment or treated directly with the specified compounds at the designated concentrations for the indicated times
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