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According to the workflow, the region of interest (all ovarian tissue) was selected under low magnification with the auto-trace assist
We drew a standard curve using the gradient dilution of the standard product as a template, added the sample, antibody and HRP aggregation reagent, and put it in a 37C water bath, then washed it five times with a washing solution, we added the chromogenic solution and reacted for 10 min, then detected the absorbance of each hole at a wavelength of 450 nm
doi: 10.3389/fendo.2014.00056 Received 20 March 2014 Accepted 07 April 2014 Published 22 April 2014 Volume 5 - 2014 Edited by Rosaria Meccariello, University of Naples Parthenope, Italy Reviewed by Rosanna Chianese, Second University of Naples, Italy