Western Blot Analysis Cellular and cerebral samples were lysed in RIPA (Beyotime, China) containing inhibitor cocktail (Roche, Germany) for 20 min, following which the lysates were centrifuged (12,000 g) at 4C for 15 min
However, you may stay on a lower dose due to preference or side effects
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Results are expressed as nmol/mg protein
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It is a reasonable supplement to a balanced diet but not a reliable substitute for a full electrolyte mix during sustained exercise or heat exposure