*The GHRH-GH-IGF-1 Axis: The brains hypothalamus signals via growth hormone releasing hormone (GHRH) to the pituitary under the brain to release growth hormone (GH), which has direct effects on muscle, bone, and fat, and stimulates the release of insulin-like growth factor-1 (IGF-1)
We propose staged translation: initial human trials using systemic IV or intranasal delivery, reserving direct CNS approaches for refractory cases
Consider the timescales
DMP IGF-I KO mice, in which the IGF-I gene is deleted in osteocytes using DMP-1 promoter-driven Cre, do not have altered serum IGF-I, calcium, or phophorus levels, but do show decreased serum levels of the bone formation maker procollagen type I N-terminal propeptide (PINP) and the bone resorption marker, c-telopeptide (CTx)
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For straightforward calculations, we recommend adding exactly 1ml of bacteriostatic water