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[7, 8] However, qualitative assays cannot provide sufficient information for females

In freshwater lakes with flows in and out via rivers, the average residence time is 1 to 100 years, while in salt lakes, from which the only exit is likely to be by evaporation, the average residence time is up to 1000 years

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RRID:Addgene_72876)) for 48 h before seeding for flux analysis at 10,000 cells per well and allowed to adhere overnight in full culture media under normal conditions