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GSH Metabolism, Biosynthesis & Health Implications (PubMed, 2004) Cysteine Availability as the Rate-Limiting Factor in GSH Synthesis A widely cited review of glutathione metabolism published on PubMed documented the two-step cytosolic biosynthesis pathway in detail, confirming that GSH synthesis is regulated primarily by gamma-glutamylcysteine synthetase activity, cysteine availability, and GSH feedback inhibition
doi: 10.1111/nep.14149 82 KoppeL.FouqueD.SoulageC
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At therapeutic doses, the small amount of NAPQI produced is neutralized by conjugation with reduced glutathione (GSH), as shown in Figure 1, a crucial tripeptide that, in an NADPH-dependent reaction, helps detoxify oxidants like NAPQI
