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After incubation, 50 L of K 3 [Fe(CN) 6 ] (2 mM) was added and the absorbance at 680 nm was recorded with a BioTek synergy 4 Multi-Mode Microplate Reader

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Unfortunately, due to many different nutritional, physiological, environmental and lifestyle factors Vitamin B12 deficiency is a common and growing problem today which may lead to anaemia and numerous unpleasant symptoms including mental fogginess, poor memory and concentration, fatigue, irritation, feelings of apathy and lack of motivation, mood swings, muscle weakness, or sleeping problems

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