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After TMB substrate solution is added, only those wells that contain Human FE, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color

The CKM gene is located on chromosome 19q13.32 and is composed of 8 exons that encode a protein of 381 amino acids

[17] However, more studies are needed to confirm this

Additionally, a significant concentration of ER is found in the genioglossus muscle, where E2 influences its contractility primarily through the ER pathway, while also promoting the expression of ER mRNA and protein, with no observed effect on ER (30)

The peptide sequence consists of fifteen amino acids arranged as Gly-Glu-Pro-Pro-Pro-Gly-Lys-Pro-Ala-Asp-Asp-Ala-Gly-Leu-Val, forming a stable molecular structure that demonstrates resistance to enzymatic degradation compared to other peptide compounds

E., Chondrogianni, N., & Knigsberg, M