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Table III: Retention times in this acylcarnitines LC-MS/MS analysis were highly consistent over 250 injections for all compounds, demonstrating good robustness

doi: 10.1371/journal.pone.0189248

All these MCFAs exerted anticancer activity by downregulating genes linked to cell division [cyclin dependent kinase 2 (CDK2), cyclin dependent kinase 4 (CDK4), CDC28 protein kinase regulatory subunit 1B (CKS1B), cyclin A2 (CCNA2), cyclin D1 (CCND1)] and upregulating the Gadd45a (growth arrest and DNA damage inducible ) gene, which plays a pivotal role in inducing apoptosis in colon cancer cells

Moreover, the C-terminal serine of the peptide was replaced by cysteine via site-directed mutagenesis performed with polyethylene glycol

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