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Researchers typically determine dosage based on Animal size (e.g., rodents vs

Mixed results

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The column was washed with 15 column volumes of PPS buffer (20 mM potassium phosphate, 200 mM NaCl, pH 7.4) at a flow rate of 5 ml min 1

Heather LC, Pates KM, Atherton HJ, Cole MA, Ball DR, Evans RD, Glatz JF, Luiken JJ, Griffin JL, Clarke K (2013) Differential translocation of the fatty acid transporter, FAT/CD36, and the glucose transporter, GLUT4, coordinates changes in cardiac substrate metabolism during ischemia and reperfusion