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PMID: 25900608

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The solution then passes through a two-stage filtration process that sterilizes and removes endotoxins

As secondary detection of the membranes, streptavidin-horseradish peroxidase (HRP) provided in each ELISA kit was used at the concentration (and diluents) suggested by the manufacturer for one hour and then washed as described above

These pleiotropic anti-inflammatory effects of DPP-4 inhibitors are incomparable to standard anti-inflammatory therapies (i.e., corticosteroids) and might be useful in the therapy of COVID-19 due to their other favorable effects, especially after the clinical findings, suggesting the lack of benefit from corticosteroids and even indicating to deleterious effects accompanied with late complications (Russel et al., 2020)