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Shortening the time from the onset of ALS symptoms to diagnosis is essential to evaluate and initiate treatments and therapeutics sooner, especially if they exert a meaningful disease-modifying effect

Afterwards, a hemi-nested PCR (FastStart High Fidelity PCR System, dNTPack, Roche) including a fragment of 388 bp in NS5B was carried out including nucleotide positions from 8254 to 8641

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In clinical practice, physicians should be proactive in asking people with type 1 diabetes whether, and at which glucose level, they feel hypoglycaemia in order to identify IAH and adjust individual glucose targets to prevent the occurrence of Level 3 hypoglycaemia

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