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doi: 10.1515/jpem-2015-0233 80 KhorECFanshaweBQiYZolotukhinSKulkarniRNEnriquezRFet al

This happens if the water was injected too rapidly (crushing the delicate peptide chains), the vial was shaken instead of gently swirled, or the solution's pH level is off

J Leukoc Biol 66:411415

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The sections were then counterstained with haematoxylin solution for 90 s and rinsed again in tap water