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Iron regulatory protein 1 outcompetes iron regulatory protein 2 in regulating cellular iron homeostasis in response to nitric oxide

Experiments performed with 5- to 320-L bed volumes packing into a 1mL pipette tip column and agarose-based protein A affinity resin.1 The scalability of PhyTip columns is demonstrated in Figure 3, which shows a clear relationship between the volume of the resin bed and the amount of protein recovered

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