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(2021) 101:adv00512

The engineered constructs were 168 amino acids in length, with native or modified GLP-1 ranging from 1 to 30

In the recording chamber, a single frontal cortex slice was submerged and perfused continuously (2.0 ml/min) with ACSF containing 124 mM NaCl, 3 mM KCl, 2 mM CaCl 2 , 2 mM MgCl 2 , 1 mM NaH 2 PO 3 , 26 mM NaHCO 3 , and 10 mM glucose equilibrated with 95% O 2 and 5% CO 2 , pH 7.357.45

Twenty-four hours later, the NAC-containing medium was washed off, and replaced with fresh medium containing the indicated FA concentration, to a final volume of 200 L per well

Safety considerations for peptide combinations Combining peptides with Ozempic requires careful attention to safety

recommendations for their implementation are given by Simone et al