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In response to decreasing ambient light, detected by the retina and relayed through the suprachiasmatic nucleus (SCN) of the hypothalamus, the pineal gland increases melatonin production, signalling the body that it is time to sleep

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With this in mind we optimized a fast and scalable single-shot analysis workflow based on high-throughput magnetic Ti-IMAC bead enrichment of phosphopeptides from 200 g of starting tryptic peptide material (Fig

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