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Notably, in the lumbar spine, prolonged CR (20 or 74 weeks) yielded graded benefits in trabecular volumetric BMD, bone volume fraction, and trabecular number

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It is also possible that while heating is effective at disrupting protein shapes and eliminating enzyme activity, it does not eliminate ice nucleation activity as the proteins are twisted into new shapes that have ice nucleation sites, similar to the hypothesis that defects and aggregation of large proteins are a source of ice nucleation sites 55

TF activity was another parameter investigated in this study