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After spinning the tubes to retire as much of the supernatant as possible, pellets were air-dried at RT for 5 min and suspended in 20 l DEPC water (Carl Roth GmbH)

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This rebound effect is why consistency matters more than total dosage for conditions like non-alcoholic fatty liver disease (NAFLD), where glutathione's hepatoprotective effects depend on sustained antioxidant tone rather than intermittent spikes

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SIRT2 activates G6PD to enhance NADPH production and promote leukaemia cell proliferation