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In the oxygen-glucose deprivation/reperfusion (OGD/R)-induced RPE injury model, excessive ROS generation triggers iron-dependent lipid peroxidation, leading to ferroptosis, while simultaneously activating the NLRP3 inflammasome
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Immune cells are particularly sensitive to oxidative stress because of their high percentage of polyunsaturated fatty acids in the plasma membranes, along with naturally producing more oxidative products
For colony formation assays, PC-3 cells were stably transfected with vectors, seeded in six-well dishes (in triplicate) (density: 2 10 3 cells per well), and allowed to adhere overnight
The highly expressed ATF6 mediates tolerance to ferroptosis through transcriptional activation of PLA2G4A, and inhibition of ATF6 signaling by Ceapin-A7 enhances the effect of enzalutamide on CRPC xenograft growth [62]
