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Once centrifuged, the samples were stored at 80C until further analysis with LCMS/MS

Light: UV exposure can break the chemical bonds holding the peptide together

However, beef insulin has not been manufactured for human use in the U.S

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Statistical analysis Data are presented as mean of at least three independent experiments and error bars denote s.e.m

Insulin resistance is the inadequate response of target tissues to the physiological effects of circulating insulin, such as decreased glucose uptake in skeletal muscles under insulin stimulation, inhibition of hepatic gluconeogenesis by insulin, and relief of the inhibitory effect of insulin on lipid breakdown in adipose tissue (Schenk et al., 2008)