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The reactions were allowed to incubate in the dark at 37 C for 30 min, during which fluorescence was measured in a SpectraMax iD3 Multi-Mode Microplate Reader (Molecular Devices, San Jose, CA, USA) using excitation in the range of 530560 nm and emission detection at 590 nm
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[14] A 2018 systematic review and meta-analysis of 12 randomized controlled trials found "supplementation significantly decreases depressive symptoms compared with placebo/no intervention, while offering a comparable effect with that of established antidepressant agents with fewer adverse effects." The review also indicates that "the effect of ALC in younger subjects was not more effective than placebo in improving these symptoms." indicating a need for more research explaining the age/effect relation
It was a quiet admission that the original model community-led, effort-driven, belief-based no longer makes sense in a world where weight loss can be outsourced to chemistry
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