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Oct 2012;188(4):1186-91

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For signal conversion, slides were incubated with 50 L of converter-POD (anti-fluorescein antibody conjugated with horseradish peroxidase) for 30 min at 37C, rinsed with PBS, and then incubated with 50 L of 3,3'-diaminobenzidine (DAB) substrate solution for 10 min at 25C