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In primary culture of satellite cells, the LASSS-treated HGF (Figure 1f, bars 9 and 11 , at 5 ng/ml without or with exposure to peroxynitrite, respectively) normally activates satellite cells to a level equivalent to the positive control ( bar 2 ) as revealed by BrdU-incorporation assay

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Eggersdorfer M et al

Establishing effective concentrations of nicotine For the purpose of establishing effective concentrations of nicotine, incubations were performed with confluent monolayer cultures of 4 of the established cell-lines of HGF in Eagle's MEM using 14C-testosterone/14C-4-androstenedione as substrate, in 24 well multiwell dishes

w, with: 4.5 g l 1 glucose, w: L-glutamine, w/o, without: sodium pyruvate, w: 3.7 g l 1 NaHCO 3 ) was purchased from PAN Biotech, high-glucose DMEM, FCS and PBS were purchased from Sigma-Aldrich

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