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Following more washing steps, tyramide signal amplification was used for the GCGR antibody using a kit according to the manufacturers instructions (Alexa Fluor 488 Tyramide Reagent
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The primary outcome was a composite kidney outcomekidney failure, sustained 40% decline in eGFR, or death from renal causes
Chronic fetal hypoglycemia inhibits the later steps of stimulus-secretion coupling in pancreatic beta-cells
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