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Gut and liver (2020) Chang CH, Tsai WC, et al

It begins with dysfunctional transmembrane insulin receptor (as a result of lipidperoxidation)

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Barnett ML, et al

The day before the assay, frozen aliquots of the above-mentioned BHK cell lines were thawed, washed once in PBS (Gibco, #14190-094) and seeded in 40 L medium in a white 384-well culture plate (PerkinElmer, #6007688) at a cell density of 4000 cells/well and incubated in plastic bags over night at 37 C and 5% CO 2