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In the presence of 5.5 mM glucose, GLP- 1-mediated increments of [Ca 2+ ] i were reduced to approximately 20% after pre-treatment with 1 mM metformin (p 100 M metformin blocked this effect in the presence of 5.5 mM glucose, no effect of metformin was observed in the presence of 16.7 mM glucose (Figure 3(a))

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Its signaling complexity (triple agonism) imposes heightened methodological vigilance: systematic glycemic monitoring to detect any paradoxical hyperglycemia linked to the glucagon arm, regular hepatic panel given the lipolytic effect, and lean mass tracking to quantify the share of weight loss linked to muscle depletion under severe caloric restriction

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The net effect is stored triglycerides getting broken apart into free fatty acids and glycerol, which the body can then burn for energy